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Product Details |
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Species Reactivity |
human, mouse, rat, pig, rabbit,canine, monkey,chicken,zebrafish, hamster |
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Published Species |
human, mouse, rat,chicken, goat,sheep, aedesalbopictus, arabidopsis, golden hamsters,cat |
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Host/Isotype |
Mouse / IgG2b |
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Class |
monoclonal |
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Type |
Antibody |
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Calculated MW |
42 kDa |
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Observed MW |
42 kDa |
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Form |
Liquid |
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Concentration |
1 mg/mL |
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Amount |
20 µg, 100 µg |
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Purification |
Protein A purification |
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Storage buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH7.3 |
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Storage conditions |
-20°C, Avoid Freeze/Thaw Cycles |
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Applications |
Tested Dilution |
Positive Controls |
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Western Blot (WB) |
1:20000-1:100000 |
HeLa cells, A549 cells, Jurkatcells, mouse pancreas, rat brain, rat pancreas, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, CHO cells, HSC-T6 cells. NIH/3T3 cells, Pig brain, Rabbit brain, Mouse brain, Chicken brain |
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Immunoprecipitation (IP) |
0.5-4.0 μg for 1.0-3.0mg oftotal protein lysate |
HeLa cells |
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Immunohistochemistry (IHC) |
1:20-1:2000 |
human kidney tissue, human brain tissue, human colon cancertissue, human heart tissue |
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Immunofluorescence on Paraffin section (IF-P) |
1:500-1:2000 |
mouse brain tissue |
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Immunocytochemistry (ICC/IF) |
1:500-1:2000 |
MDCK cells, HeLa cells |
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Flow Cytometry (intracellular) |
0.40 µg per 10^6 cellsin a 100 µL suspension |
HeLa cells |
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ELISA (ELISA) |
- |
- |
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Miscellaneous PubMed (Misc) |
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Background Information
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
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