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Product Details |
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Species Reactivity |
human, mouse, rat, pig |
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Published Species |
human, mouse, rat, pig, canine, monkey, chicken,bovine, goat |
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Host/Isotype |
Rabbit / IgG |
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Class |
monoclonal |
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Type |
Antibody |
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Calculated MW |
375 aa, 42 kDa |
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Observed MW |
42 kDa |
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Form |
Liquid |
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Concentration |
1 mg/mL |
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Amount |
20 µg, 100 µg |
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Purification |
Protein A purification |
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Storage buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH7.3 |
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Storage conditions |
-20°C, Avoid Freeze/Thaw Cycles |
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Applications |
Tested Dilution |
Positive Controls |
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Western Blot (WB) |
1:5000-1:50000 |
HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
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Immunoprecipitation (IP) |
0.5-4.0 μg for 1.0-3.0 mg of total protein lysate |
HeLa cells |
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Immunohistochemistry (IHC) |
1:1000-1:4000 |
rat colon tissue |
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Immunocytochemistry (ICC/IF) |
1:950-1:3800 |
U2OS cells, MDCK cells |
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Flow Cytometry (Flow) |
- |
- |
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ELISA (ELISA) |
- |
- |
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Miscellaneous PubMed (Misc) |
- |
- |
Background Information
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
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