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Product Details |
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Species Reactivity |
human, mouse, rat |
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Published Species |
human, mouse, rat |
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Host/Isotype |
Rabbit / IgG |
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Class |
monoclonal |
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Type |
Antibody |
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Calculated MW |
36 kDa |
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Observed MW |
36 kDa |
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Form |
Liquid |
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Concentration |
1 mg/mL |
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Amount |
20 µg, 100 µg |
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Purification |
Protein A purification |
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Storage buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH7.3 |
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Storage conditions |
-20°C, Avoid Freeze/Thaw Cycles |
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Applications |
Tested Dilution |
Positive Controls |
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Western Blot (WB) |
1:5000-1:50000 |
mouse brain tissue, rat brain tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurakat cells, HEK-293 cells, HeLa cells |
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Immunoprecipitation (IP) |
- |
- |
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Immunohistochemistry (IHC) |
- |
- |
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Immunocytochemistry (ICC/IF) |
1:500-1:2000 |
HeLa cells |
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Flow Cytometry (Flow) |
- |
- |
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ELISA (ELISA) |
- |
- |
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Miscellaneous PubMed (Misc) |
- |
- |
Background Information
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
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