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Product Details |
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Species Reactivity |
human, mouse, rat, pig,zebrafish, yeast |
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Published Species |
human, rat,chicken, goat, yeast, deer, whiteflies, tick,treeshrew,cynomorium songaricum |
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Host/Isotype |
Mouse / IgG2b |
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Class |
monoclonal |
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Type |
Antibody |
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Calculated MW |
36 kDa |
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Observed MW |
36 kDa |
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Form |
Liquid |
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Concentration |
1 mg/mL |
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Amount |
20 µg, 100 µg |
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Purification |
Protein A purification |
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Storage buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH7.3 |
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Storage conditions |
-20°C, Avoid Freeze/Thaw Cycles |
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Applications |
Tested Dilution |
Positive Controls |
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Western Blot (WB) |
1:50000-1:500000 |
HeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybeanwhole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkatcells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue |
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Immunoprecipitation (IP) |
0.5-4.0μg for 1.0-3.0mg oftotal protein lysate |
HeLa Cells |
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Immunohistochemistry (IHC) |
1:3000-1:16000 |
human livercancertissue |
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Immunocytochemistry (ICC/IF) |
1:400-1:1600 |
HeLa cells |
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Flow Cytometry (intracellular) |
0.40μg per 10^6 cellsin a 100μL suspension |
HeLa cells |
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ELISA (ELISA) |
- |
- |
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Miscellaneous PubMed (Misc) |
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Background Information
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
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